Testicular or epididymal sperm aspiration is a technique that can be added to in vitro fertilization, oocyte donation or gestational surrogacy.
The most common conditions requiring sperm aspiration are:
- Previous vasectomy (with or without an attempt at reversal)
- Congenital absence of vas deferens (the connecting tube between testicles and penis)
- Sperm concentration and/or sperm quality so low that no normal sperm can be found in the ejaculate
Once sperm are successfully aspirated, ICSI (Intracytoplasmic Sperm Injection) procedure is used for fertilization of eggs obtained through in vitro fertilization, oocyte donation or gestational surrogacy.
In ICSI, a single sperm is inserted into an egg under a microscope using micro-instruments.

The sperm aspiration can be scheduled for the morning of egg retrieval procedure and the sperm is use for the ICSI the same day. Any sperm left over can normally be cryopreserved and stored for potential subsequent use.
Alternatively, the sperm aspiration can be done prior to the egg retrieval. The collected sperm is frozen and stored in liquid nitrogen. The sperm sample is then thawed the morning of the egg retrieval and used for ICSI fertilization.
For additional in-depth information, please see Nova Treatments and Fees page.
If you wish to schedule an appointment for the sperm aspiration treatment, please go to New Patient Appointment at Nova.
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